Asan Medical Center 4 articles published in JoVE Biology A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells Jeong Hoon Lee1, Jeong Mi Ha1, Quynh Mai Ho1, Chae Hun Leem1,2,3 1Department of Physiology, University of Ulsan College of Medicine/Asan Medical Center, 2Asan Medical Center, 3Asan Medical Institute of Convergence Science and Technology Due to the spectral overlapping of the excitation and emission wavelengths of NADH and fura-2 analogs, the signal interference from both chemicals in live cells is unavoidable during quantitative measurement of [Ca2+]. Thus, a novel online correction method of NADH signal interference to measure [Ca2+] was developed. Immunology and Infection Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation Jun-O Jin1,2, Soyeong Jang3, Hyehyun Kim4, Junghwan Oh4, Sungbo Shim5, Minseok Kwak3,4, Peter C.W. Lee6 1Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, 2Department of Medical Biotechnology, Yeungnam University, 3Department of Chemistry, Pukyong National University, 4Marine-integrated Bionics Research Center, Pukyong National University, 5Department of Biochemistry, Chungbuk National University, 6Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center Experimental procedures for the subsequent extraction of lymphatic tissues to test lymphoid dendritic cell activation are described after treatment of an immunostimulating nanomaterial. Biology Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy Min Kyo Jung1, Ji Young Mun2 1Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences, Asan Medical Center, 2Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network, Korea Brain Research Institute This protocol describes the various techniques necessary for transmission electron microscopy including negative staining, ultrathin sectioning for detailed structure, and immuno-gold labelling to determine the positions of specific proteins in exosomes. Genetics RNA Interference-based Investigation of the Function of Heat Shock Protein 27 during Corneal Epithelial Wound Healing Aeri Yoo*1, Hyun-Min Park*2, Soon-Suk Kang2, Eun-Soon Kim2, Hungwon Tchah2, Jae Yong Kim2 1Department of Ophthalmology, Saevit Eye Hospital, 2Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center Herein, we present a protocol to use heat shock protein 27 (HSP27)-specific small interfering RNA to assess the function of HSP27 during corneal epithelial wound healing. RNA interference is the best method for effectively knocking-down gene expression to investigate protein function in various cell types.
