Gwangju Women's University 1 article published in JoVE Genetics An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness Seoghyun Lee*1, Minho Won*2, Ran Hee Hwang3, Gang Min Hur2, Hyunju Ro1 1Department of Biological Sciences, College of Bioscience and Biotechnology, Chungnam National University, 2Department of Pharmacology, College of Medicine, Chungnam National University, 3 Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment.