Korea Institute of Science and Technology (KIST)

10 articles published in JoVE

• Biochemistry

  
  Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes Dohwan Lee*¹, Kyung Wook Wee*¹, Hyerin Kim*¹, Sung Il Han¹, Seungmin Kwak^1,2, Dae Sung Yoon³, Jeong Hoon Lee^{1 1}Department of Electrical Engineering, Kwangwoon University, ²Korea Institute of Science and Technology (KIST), ³School of Biomedical Engineering, Korea University Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and exosomes.

• Medicine

  
  Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans Tram Anh Ngoc Le¹, Baskar Selvaraj^1,2, Jae Wook Lee^1,2, Kyungsu Kang^{1,2 1}Gangneung Institute of Natural Products, Korea Institute of Science and Technology, ²Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST) This protocol describes how to measure intestinal permeability of Caenorhabditis elegans. This method is helpful for basic biological research on intestinal health related to the interaction between intestinal bacteria and their host and for screening to identify probiotic and chemical agents to cure leaky gut syndrome and inflammatory bowel diseases.

• Bioengineering

  
  Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties Youngmin Seo*¹, Chanhwi Park*², Jaewoo Son², Kyungwoo Lee², Jangsun Hwang², Yeonho Jo², Dohyun Lee², Muhammad Saad Khan², Sachin Ganpat Chavan², Yonghyun Choi², Dasom Kim², Assaf A Gilad³, Jonghoon Choi^{2 1}Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, ²School of Integrative Engineering, Chung-Ang University, ³Division of Synthetic Biology and Regenerative Medicine, Institute for Quantitative Health Science and Engineering, Michigan State University In this study, antimicrobial nanomaterials were synthesized by acidic oxidation of multiwalled carbon nanotubes and subsequent reductive deposition of silver nanoparticles. Antimicrobial activity and cytotoxicity tests were performed with the as-prepared nanomaterials.

• Neuroscience

  
  Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus Anvar Sariev^1,2, Jinho Chung¹, Dajung Jung^1,3, Farnaz Sharif^1,2, Ju-Young Lee^1,2, Soyoun Kim¹, Sebastien Royer^{1,2 1}Center for Functional Connectomics, Korea Institute of Science and Technology, ²Biomedical department, KIST school, University of Science and Technology, ³Department of Biological Sciences, Korea Advanced Institute of Science and Technology We describe the diverse steps to implant chronic silicon probes and to record place cells in mice that are running head-fixed on a cue-enriched treadmill apparatus.

• Medicine

  
  Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans So Young Lee¹, Kyungsu Kang^{1 1}Systems Biotechnology Research Center, Korea Institute of Science and Technology A basic protocol to evaluate the toxicity of chemicals in a model animal, Caenorhabditis elegans, is described. The method is convenient and useful for the development of pharmaceuticals as well as for the risk assessment of various environmental pollutants.

• Bioengineering

  
  Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone Minyoung Kim^1,2, Hyunjoon Rhee^1,3, Ji Yoon Kang¹, Tae Song Kim¹, Rhokyun Kwak^{1 1}Center for BioMicrosystems, Korea Institute of Science and Technology, ²Department of Mechanical Engineering, Seoul National University, ³Department of Industrial Engineering, University of Illinois Urbana-Champaign The protocol for a novel ion concentration polarization (ICP) platform that can stop the propagation of the ICP zone, regardless of the operating conditions is described. This unique ability of the platform lies in the use of merging ion depletion and enrichment, which are two polarities of the ICP phenomenon.

• Medicine

  
  Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli Dong-Eog Kim¹, Jeong-Yeon Kim¹, Su-Kyoung Lee¹, Ju Hee Ryu², Ick Chan kwon², Cheol-Hee Ahn³, Kwangmeyung Kim², Dawid Schellingerhout^{4 1}Molecular Imaging and Neurovascular Research Laboratory, Dongguk University College of Medicine, ²Biomedical Research Center, Korea Institute of Science and Technology, ³Research Institute of Advanced Materials, Department of Materials Science and Engineering, Seoul National University, ⁴Departments of Radiology and Cancer Systems Imaging, University of Texas M.D. Anderson Cancer Center This protocol describes the application of combined near-infrared fluorescent (NIRF) imaging and micro-computed tomography (microCT) for visualizing cerebral thromboemboli. This technique allows the quantification of thrombus burden and evolution. The NIRF imaging technique visualizes fluorescently labeled thrombus in excised brain, while the microCT technique visualizes thrombus inside living animals using gold-nanoparticles.

• Neuroscience

  
  Intracerebroventricular Injection of Amyloid-β Peptides in Normal Mice to Acutely Induce Alzheimer-like Cognitive Deficits Hye Yun Kim^1,2, Dongkeun K. Lee¹, Bo-Ryehn Chung^1,3, Hyunjin V. Kim^1,4, YoungSoo Kim^{1,4 1}Center for Neuro-Medicine, Korea Institute of Science and Technology, ²Research Institute, GoshenBiotech, Inc., ³Department of Chemical and Biological Engineering, Princeton University, ⁴Biological Chemistry Program, Korea University of Science and Technology The amyloid-β (Aβ)-injected animal model enables the administration of a defined quantity and species of Aβ fragments and reduces individual differences within each study group. This protocol describes the intracerebroventricular (ICV) injection of Aβ without stereotactic instruments, enabling the production of Alzheimer-like behavioral abnormalities in normal mice.

• Neuroscience

  
  Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors Sungmoo Lee^1,2, Hong Hua Piao², Masoud Sepheri-Rad², Arong Jung^2,3, Uhna Sung², Yoon-Kyu Song^1,4, Bradley J. Baker^{2 1}Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, ²Center for Functional Connectomics, Korea Institute of Science and Technology, ³College of Life Sciences and Biotechnology, Korea University, ⁴Advanced Institutes of Convergence Technology A method for imaging changes in membrane potential using genetically encoded voltage indicators is described.

• Neuroscience

  
  High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode Mina Lee^1,2, Dongwook Kim^1,2, Hee-Sup Shin^1,2, Ho-Geun Sung³, Jee Hyun Choi^{1,2 1}Center for Neural Science , Korea Institute of Science and Technology (KIST), ²Department of Neuroscience, University of Science and Technology, ³Fab Service Department, Korea Advanced Nano Fab Center In this article, we described the surgery procedure and handling tips for implantation of ultra-thin polyimide-based microelectrode array (PBM-array) on the mouse skull for acquisition of high-density encephalography (EEG) in a mouse model.