University of the Punjab 1 article published in JoVE Biology Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain Hannah Levengood1, Yanxia Dou1, Jinping Fan1, Anna Bajszar2, Jing Huang1, Syed Mohsin Abbas3, Yun Zhou4, Cankui Zhang1 1Department of Agronomy, Center for Plant Biology, Purdue University, 2Department of Biology, Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences, University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University Because of its versatile application as a model species in various fields of study, there is a need for a genetic transformation toolkit in narrowleaf plantain (Plantago lanceolata). Here, using Agrobacterium tumefaciens-mediated transformation, a protocol is presented that results in stable transgenic lines with a transformation efficiency of 20%.
