2 articles published in JoVE
Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography Sophie Sosnovskaya*1,2, Amir N. Zakirov*1,2, Ekaterina D. Ryumina*1,3, Ekaterina Kharybina1,2, Sergei A. Golyshev1, Olga S. Strelkova1, Oxana A. Zhironkina1, Andrei Moiseenko2, Anton Orekhov4, Igor I. Kireev1,2,5 1Belozersky Institute of Physico-chemical Biology, Lomonosov Moscow State University, 2Faculty of Biology, Lomonosov Moscow State University, 3Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 4National Research Center, Kurchatov Institute, 5V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology This protocol presents a technique for high-resolution mapping of replication sites in structurally preserved chromatin in situ that employs a combination of pre-embedding EdU-streptavidin-Nanogold labeling and ChromEMT.
Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae Ivan V. Chicherin1,2, Sergei A. Levitskii1, Maria V. Baleva1, Igor A. Krasheninnikov1, Maxim V. Patrushev3, Piotr A. Kamenski1 1Department of Biology, M.V. Lomonosov Moscow State University, 2Institute of Functional Genomics, M.V. Lomonosov Moscow State University, 3National Research Centre "Kurchatov Institute" Baker’s yeast mitochondrial genome encodes eight polypeptides. The goal of the current protocol is to label all of them and subsequently visualize them as separate bands.