3 articles published in JoVE
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum Abhijeet Patra1, Tao Ding2, Minghui Hong3, Arthur Mark Richards2, Ten It Wong4, Xiaodong Zhou4, Chester Lee Drum2 1NUS Nanoscience and Nanotechnology Initiative, National University of Singapore, 2Cardiovascular Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, 3Department of Electrical and Computer Engineering, National University of Singapore, 4Institute of Materials Research Engineering, A*STAR (Agency for Science, Technology and Research) This work describes a nanoimprinting lithography method to fabricate high-quality sensing arrays that work on the principle of extraordinary optical transmission. The biosensor is low-cost, robust, easy to use, and can detect cardiac troponin I in serum at clinically relevant concentrations (99th percentile cutoff ∼10-400 pg/mL, depending on the assay).
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes Wen Jing Sim1, Frano Malinarich1, Anna-Marie Fairhurst2, John Edward Connolly1,3 1Translational Immunology, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 2Singapore Immunology Network, 3Institute of Biomedical Studies, Baylor University Immature dendritic cells can be selectively differentiated into tolerogenic or mature dendritic cells to regulate the balance between immunity and tolerance. This work presents a means to generate from immature monocyte derived dendritic cells (moDCs), in vitro tolerogenic and mature moDCs that differ in metabolic phenotypes.
Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation Yufen Goh*1, Melissa J. Fullwood*1,2,3, Huay Mei Poh1, Su Qin Peh1, Chin Thing Ong1, Jingyao Zhang1, Xiaoan Ruan1, Yijun Ruan1,3 1Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, 2A*STAR-Duke-NUS Neuroscience Research Partnership, Singapore, 3Department of Biochemistry, National University of Singapore, Singapore Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET) is a method for de novo detection of chromatin interactions, for better understanding of transcriptional control.