Dogus University View Institution's Website 1 article published in JoVE Biology An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications Huseyin C. Yalcin1,2, Akshay Shekhar1, Ajinkya A. Rane1, Jonathan T. Butcher1 1Department of Biomedical Engineering, Cornell University, 2Current Address: Mechanical Engineering Department, Dogus University In this article, we present a simple methodology to enable long-term ex-ovo avian embryo culture. This technique is ideal for longitudinal experimentation requiring complete optical accessibility and/or sterile transportation in avian embryos.