3 articles published in JoVE
Extraction and Analysis of Taiwanese Green Propolis Chun-Ting Chen1, Yi-Hsuan Chien1, Yu-Hsiang Yu1, Yue-Wen Chen1 1Department of Biotechnology and Animal Science, National Ilan University We present a protocol for using ethanol as a solvent to extract and characterize Taiwanese green propolis that exhibits antibacterial activity.
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay Ju-Chun Chang1, Se Jin Lee2, Jae Su Kim2, Chung-Hsiung Wang3, Yu-Shin Nai1 1Department of Biotechnology and Animal Science, National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, 3Department of Entomology, National Taiwan University This protocol describes a heat shock-induced protein expression system (pDHsp/V5-His/sf9 cell system), which can be used for either expressing foreign proteins or evaluating the anti-apoptotic activity of potential foreign proteins and their truncated amino acids in insect cells.
Evaluating the Effect of Environmental Chemicals on Honey Bee Development from the Individual to Colony Level Chong-Yu Ko1, Yue-Wen Chen1, Yu-Shin Nai1 1Department of Biotechnology and Animal Science, National Ilan University Herein we present a method to feed pesticide contaminated food to both an individual honey bee and a beehive colony. The procedure evaluates the pesticide effect on individual honey bees by in vivo feeding of basic larval diet and also on the natural condition of beehive colony.