National Sun Yat-sen University

5 articles published in JoVE

• Biology

  
  Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique Ke-Rui Chou¹, HsinYuan Tsai^{1,2 1}Department of Marine Biotechnology and Resources, National Sun Yat-sen University, ²Doctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University The present protocol developed a method to estimate the yield of compounds on the TLC plate using the blue-LED illumination technique. The advantages of this approach are that it is safe, effective, inexpensive, and allows the researcher to measure multiple samples simultaneously.

• Immunology and Infection

  
  Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining Liang-Chun Wang¹, Jacob Wagner², Annabelle Capino², Elizabeth Nesbit², Wenxia Song², Daniel C. Stein^{2 1}Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, ²Department of Cell Biology and Molecular Genetics, University of Maryland A simple ATP-measuring assay and live/dead staining method were used to quantify and visualize Neisseria gonorrhoeae survival after treatment with ceftriaxone. This protocol can be extended to examine the antimicrobial effects of any antibiotic and can be used to define the minimal inhibitory concentration of antibiotics in bacterial biofilms.

• Medicine

  
  Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats Hsin-Ting Lin^1,2, Yi-Lin Shiou¹, Wun-Jyun Jhuang¹, Hsiang-Chun Lee^{1,3,4,5,6 1}Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, ²Hematology and Oncology, China Medical University Hospital, ³Department of Internal Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, ⁴Center for Lipid Biosciences, Kaohsiung Medical University Hospital, ⁵Lipid Science and Aging Research Center, Kaohsiung Medical University, ⁶Institute/Center of Medical Science and Technology, National Sun Yat-sen University Here, we describe a setup for simultaneous recording of electrocardiography and intra-arterial blood pressure (BP) in experimental rats, which can be done with standard equipment in animal facilities and can be applied to physiological or pharmacological studies to investigate pathogenic or therapeutic mechanisms in cardiovascular medicine.

• Environment

  
  Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues Wen-Ta Li¹, Bang-Yeh Liou¹, Wei-Cheng Yang², Meng-Hsien Chen³, Hui-Wen Chang^1,2, Hue-Ying Chiou⁴, Victor Fei Pang^1,2, Chian-Ren Jeng^{1,2 1}Graduate Institute of Molecular and Comparative Pathobiology, National Taiwan University, ²School of Veterinary Medicine, National Taiwan University, ³Department of Oceanography and Asia-Pacific Ocean Research Center, National Sun Yat-sen University, ⁴Graduate Institute of Veterinary Pathobiology, National Chung Hsing University A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues.

• Environment

  
  Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies Kuo-Tung Jiann*¹, Liang-Saw Wen*^2,3, Peter H. Santschi^{4 1}Department of Oceanography, National Sun Yat-sen University, ²Institute of Oceanography, National Taiwan University, ³Taiwan Ocean Research Institute, National Research Laboratories, ⁴Department of Oceanography and Marine Sciences, Texas A&M University at Galveston Special care using "clean techniques" is required to properly collect and process water samples for trace metal studies in aquatic environments. A protocol for sampling, processing, and analytical procedures with the aim of obtaining reliable environmental monitoring data and results with high sensitivity for detailed trace metal studies is presented.