Bio21 Institute 1 article published in JoVE Biology Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia Perran A. Ross1, Jason K. Axford1, Kelly M. Richardson1, Nancy M. Endersby-Harshman1, Ary A. Hoffmann1 1School of BioSciences, Bio21 Institute and University of Melbourne Aedes aegypti mosquitoes infected with Wolbachia are being released into natural populations to suppress the transmission of arboviruses. We describe methods to rear Ae. aegypti with Wolbachia infections in the laboratory for experiments and field release, taking precautions to minimize laboratory adaptation and selection.
