Burnet Institute 4 articles published in JoVE Chemistry Detection and Quantification of Plasmodium falciparum in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis Miguela Martin1, David Perez-Guaita1, Dean W. Andrew3, Jack S. Richards3,4, Bayden R. Wood1, Philip Heraud1,2 1Centre for Biospectroscopy, Monash University, 2Department of Microbiology, Faculty of Medicine, Nursing and Health Sciences, Monash University, 3Centre for Biomedical Research, Burnet Institute, 4Department of Medicine, University of Melbourne Here, we present a protocol for the detection and quantification of Plasmodium falciparum in infected aqueous red blood cells using an attenuated total reflection infrared spectrometer and multivariate data analysis. Immunology and Infection Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions Thomas A. Angelovich1,2, Anna C. Hearps1,3, Anna Maisa1, Theodoros Kelesidis4, Anthony Jaworowski1,3 1Centre for Biomedical Research, Burnet Institute, 2School of Health and Biomedical Sciences, RMIT University, 3Department of Infectious Diseases, Monash University, 4University of California, Los Angeles We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity. Immunology and Infection Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein Shubhendu Sen Roy1, Huy Cong Xuan Nguyen1, Thomas A. Angelovich2,3, Anna C. Hearps2, Diana Huynh1,4, Anthony Jaworowski2,4, Theodoros Kelesidis1 1University of California, Los Angeles, 2Centre for Biomedical Research, Burnet Institute, 3School of Health and Biomedical Sciences, RMIT University, 4Department of Infectious Diseases, Monash University We describe here a fluorometric cell-free biochemical assay for determination of HDL-lipid peroxidation. This rapid and reproducible assay can be used to determine HDL function in large scale studies and can contribute to our understanding of HDL function in human disease. Immunology and Infection A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood Clovis S. Palmer1,2,3, Joshua J. Anzinger4, Tiffany R. Butterfield4, Joseph M. McCune5, Suzanne M. Crowe1,2,6 1Centre for Biomedical Research, Macfarlane Burnet Institute for Medical Research and Public Health, 2Department of Infectious Diseases, Monash University, 3Department of Microbiology and Immunology, University of Melbourne, 4Department of Microbiology, The University of the West Indies, 5Division of Experimental Medicine, Department of Medicine, University of California, San Francisco, 6Department of Medicine, Monash University Monocytes are integral components of the human innate immune system that rely on glycolytic metabolism when activated. We describe a flow cytometry protocol to measure glucose transporter expression and glucose uptake by total monocytes and monocyte subpopulations in fresh whole blood.