Centre for Cancer Biology 1 article published in JoVE Neuroscience Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration Miguel Tillo1, Quenten Schwarz1,2, Christiana Ruhrberg1 1UCL Institute of Ophthalmology, University College London, 2Department of Human Immunology, Centre for Cancer Biology, South Australia Embryonic neurons are born in the ventricular zone of the neural tube, but migrate to reach appropriate targets. Facial branchiomotor (FBM) neurons are a useful model to study neuronal migration. This protocol describes the wholemount ex vivo culture of mouse embryo hindbrains to investigate mechanisms that regulate FBM migration.