Center for Vascular Research 1 article published in JoVE Immunology and Infection Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins N. Bishara Marzook*1, Dean J. Procter*1, Helena Lynn1, Yui Yamamoto2, Jacquelyn Horsington3, Timothy P. Newsome1 1School of Molecular Bioscience, University of Sydney, 2Department of Medicine, Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science, University of Melbourne A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.