University of South Australia View Institution's Website 3 articles published in JoVE Medicine Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis Benson U.W. Lei1,2, Miko Yamada1, Van L.T. Hoang3, Paul J. Belt4, Mark H. Moore5, Lynlee L. Lin2, Ross Flewell-Smith1, Nhung Dang1,2, Shoko Tomihara2, Tarl W. Prow1,2 1Future Industries Institute, University of South Australia, 2Faculty of Medicine, University of Queensland, 3Faculty of Health, Queensland University of Technology, 4Department of Plastic and Reconstructive Surgery, Princess Alexandra Hospital, 5Australian Craniofacial Unit, Women's and Children's Hospital In this article, we demonstrate how the absorbent microbiopsy technique is performed and how the sample can be used for RNA extraction for simple and simultaneous sampling of skin and blood in a minimally invasive manner. Immunology and Infection Measuring the 50% Haemolytic Complement (CH50) Activity of Serum Maurizio Costabile1 1School of Pharmacy and Medical Sciences, University of South Australia The classical pathway is activated by antibody and culminates in target cell lysis. The CH50 assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH50 of a serum sample, the calculations and interpreting of results. Immunology and Infection Determining the Reactivity and Titre of Serum using a Haemagglutination Assay Maurizio Costabile1 1School of Pharmacy and Medical Sciences, University of South Australia Haemagglutination is a form of agglutination where antibodies bind to red blood cells. Red blood cells are both readily available and the results are readily observable using the naked eye. This video demonstrates the steps involved in a haemagglutination assay, interpreting the results and determining the titre.
