3 articles published in JoVE
Techniques for Investigating the Anatomy of the Ant Visual System Fiorella Ramirez-Esquivel1, Willi A. Ribi1, Ajay Narendra2 1Research School of Biology, Australian National University, 2Department of Biological Sciences, Macquarie University This article outlines a suite of techniques in light and electron microscopy to study the internal and external eye anatomy of insects. These include several traditional techniques optimized for work on ant eyes, detailed troubleshooting, and suggestions for optimization for different specimens and regions of interest.
Exploring Deep Space - Uncovering the Anatomy of Periventricular Structures to Reveal the Lateral Ventricles of the Human Brain Alexandru S. Colibaba*1, Aicee Dawn B. Calma*1, Alexandra L. Webb1, Krisztina Valter1 1Medical School, College of Medicine, Biology and Environment, Australian National University This paper demonstrates the effective use of a fiber dissection method to reveal the superficial white matter tracts and periventricular structures of the human brain, in three-dimensional space, to aid student comprehension of ventricular morphology.
The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation Benjamin J. C. Quah1, Christopher R. Parish1 1Department of Immunology, John Curtin School of Medical Research, Australian National University CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.