3 articles published in JoVE
Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms Daniel Omileke1, Steven Bothwell1, Daniel J. Beard1, Nikolce Mackovski1, Sara Azarpeykan1, Kirsten Coupland1, Adjanie Patabendige1, Neil Spratt1,2 1School of Biomedical Sciences and Pharmacy, University of Newcastle, 2Department of Neurology, John Hunter Hospital, Hunter New England Local Health District This article describes two methods of whole-body short-duration hypothermia induction in rats. The first, rapid induction method, employs active cooling using fans and ethanol spray for a rapid decrease in temperature. The second method is a gradual cooling method. This is achieved using the combination of isoflurane anesthesia and the reduction of temperature settings on the homeothermic heat mat. This results in a gradual decrease in core body temperature without the use of any external cooling devices.
A Simple Migration/Invasion Workflow Using an Automated Live-cell Imager Xiajie Zhang1,2, Brianna C. Morten1,2, Rodney J. Scott1,2,3, Kelly A. Avery-Kiejda1,2 1Medical Genetics, Hunter Medical Research Institute, 2Priority Research Centre for Cancer Research, Innovation and Translation, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, 3Pathology North, John Hunter Hospital The current protocol describes an integrated method investigating cancer cell migration and invasion on a single platform in real-time, providing an easily reproducible and time-efficient option to study cell mobility and morphology.
Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines Brianna C. Morten1,2, Rodney J. Scott1,2,3, Kelly A. Avery-Kiejda1,2 1Medical Genetics, Hunter Medical Research Institute, 2Priority Research Centre for Cancer, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, 3Pathology North, John Hunter Hospital This protocol describes the use of three different methods for analyzing cell proliferation in breast cancer cell lines. This includes the use of conventional cell counting, luminescence-based cell viability, and cell counting through the use of a cell imager. Each offers advantages for the reproducible measurement of cell proliferation.