Victor Chang Cardiac Research Institute 3 articles published in JoVE Biology Anesthesia and Intubation of Preadolescent Mouse Pups for Cardiothoracic Surgery Jianxin Wu*1, Amy M. Nicks1,2, Justin J. Skowno3,4, Michael P. Feneley1,2, Robert M. Graham1,2, Siiri E. Iismaa*1,2 1Molecular Cardiology and Biophysics Division, Victor Chang Cardiac Research Institute, 2St Vincent’s Clinical School, University of New South Wales Sydney, 3Department of Anaesthesia, The Children’s Hospital at Westmead, 4School of Child and Adolescent Health, University of Sydney Cardiothoracic surgical models in mice >7 days old require intubation, but this is challenging for preadolescent (8-14-day-old) mouse pups and there is little information on anesthetic regimens for intubation. Here, we present dosage regimens of ketamine/xylazine/atropine in 10-day-old C57BL/6J mouse pups that allow endotracheal intubation, while minimizing animal mortality. Biology Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells Yoshika Janapala*1, Katrina Woodward*1, Jiwon Lee2, Melanie Rug2, Thomas Preiss1,3, Nikolay E. Shirokikh1 1Division of Genome Sciences and Cancer, The John Curtin School of Medical Research, The Australian National University, 2Centre for Advanced Microscopy, The Australian National University, 3Victor Chang Cardiac Research Institute We present a technique to rapidly stabilize translational (protein biosynthesis) complexes with formaldehyde crosslinking in live yeast and mammalian cells. The approach enables dissecting transient intermediates and dynamic RNA:protein interactions. The crosslinked complexes can be used in multiple downstream applications such as in deep sequencing-based profiling methods, microscopy, and mass-spectrometry. Developmental Biology Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol Hananeh Fonoudi*1,2,3,8, Hassan Ansari*1,8, Saeed Abbasalizadeh1, Gillian M Blue6,7, Nasser Aghdami1, David S Winlaw6,7, Richard P Harvey2,3,4, Alexis Bosman*2,3, Hossein Baharvand*1,5 1Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, 2Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, 3St. Vincent´s Clinical School, Faculty of Medicine, University of New South Wales, 4School of Biotechnology and Biomolecular Sciences, University of New South Wales, 5Department of Developmental Biology, University of Science and Culture, 6Heart Centre for Children, The Children´s Hospital at Westmead, 7Sydney Medical School, University of Sydney, 8Department of Developmental Biology, University of Science and Culture, Tehran, Iran Here, we present a robust, fast and scalable cardiomyocyte differentiation protocol for human pluripotent stem cells (hPSCs). Cardiomyocytes derived using this large-scale method can provide sufficient cell numbers for their effective use in human cardiovascular disease modeling, high-throughput drug screening, and potentially clinical applications.
