Queensland Children' Hospital 1 article published in JoVE Cancer Research Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma Giulia Pericoli*1, Roberta Ferretti*1, Andrew S. Moore2,3, Maria Vinci1 1Department of Onco-hematology, Gene and Cell Therapy, Bambino Gesù Children's Hospital, IRCCS, 2 This study presents a protocol of live-3D-cell immunocytochemistry applied to a pediatric diffuse midline glioma cell line, useful to study in real-time the expression of proteins on the plasma membrane during dynamic processes like 3D cell invasion and migration.
