Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA) 1 article published in JoVE Bioengineering Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells Nicolas Werschler1,2, Josef Penninger1,2,3,4 1School of Biomedical Engineering, University of British Columbia, 2Life Sciences Institute, University of British Columbia, 3Department of Medical Genetics, University of British Columbia, 4Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA) This protocol describes the generation of self-organizing blood vessels from human pluripotent and induced pluripotent stem cells. These blood vessel networks exhibit an extensive and connected endothelial network surrounded by pericytes, smooth muscle actin, and a continuous basement membrane.
