CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences 1 article published in JoVE Biochemistry High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies Sagar Raturi1, Huanyu Li1, Yung-Ning Chang2, Andreea Scacioc1, Tina Bohstedt1, Alejandra Fernandez-Cid1, Adam Evans1, Patrizia Abrusci1, Abilasha Balakrishnan1, Tomas C. Pascoa1, Didi He1, Gamma Chi1, Nanki Kaur Singh1, Mingda Ye1, Anna Li1, Leela Shrestha1, Dong Wang1, Eleanor P. Williams1, Nicola A. Burgess-Brown1, Katharina L. Dürr1, Vera Puetter2, Alvaro Ingles-Prieto3, David B. Sauer1 1Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, 2Nuvisan ICB GmbH, 3CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Structural and biochemical studies of human membrane transporters require milligram quantities of stable, intact, and homogeneous protein. Here we describe scalable methods to screen, express, and purify human solute carrier transporters using codon-optimized genes.
