Celyad 1 article published in JoVE Cancer Research A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells Gray Kueberuwa1, Weiming Zheng1, Milena Kalaitsidou1, David E. Gilham1,2, Robert E. Hawkins1 1Manchester Cancer Research Centre Building, Department Cancer Sciences, University of Manchester, 2Celyad Here, we present a protocol for the production and pre-clinical testing of murine CD19 CAR T cells by retroviral transduction and utilization as a therapy against established syngeneic A20 B-cell lymphoma in BALB/c mice with or without lymphodepleting pre-conditioning.