University Hospital and University of Bern 8 articles published in JoVE Neuroscience Measuring the Influence of Magnetic Vestibular Stimulation on Nystagmus, Self-Motion Perception, and Cognitive Performance in a 7T MRT Gerda Wyssen1,6, Miranda Morrison2, Athanasia Korda2, Wilhelm Wimmer2,3, Jorge Otero-Millan4, Matthias Ertl1, Andreas A. Szukics1,6, Thomas Wyss2, Franca Wagner5,6, Marco D. Caversaccio2,3, Georgios Mantokoudis2,6, Fred W. Mast1,6 1Department of Psychology, University of Bern, 2Department of Otorhinolaryngology, Head and Neck Surgery, lnselspital, University Hospital Bern and University of Bern, 3Hearing Research Laboratory, ARTORG Center for Biomedical Engineering Research, University of Bern, 4Herbert Wertheim School of Optometry and Vision Science, University of California, 5University Department of Diagnostic and Interventional Neuroradiology, Inselspital, Bern University Hospital, University of Bern, 6Translational Imaging Center (TIC), Swiss Institute for Translational and Entrepreneurial Medicine In this article, we describe the experimental setup, material, and procedures to assess reflexive eye movements, self-motion perception, and cognitive tasks under magnetic vestibular stimulation, as well as the anatomical orientation of the vestibular organs, in a 7 Tesla Magnetic resonance tomography (7T-MRT) scanner. Neuroscience Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit Stefan Wanderer1,2, Claudia Waltenspuel2, Basil E. Grüter1,2, Fabio Strange1,2, Sivani Sivanrupan2, Luca Remonda3, Hans Rudolf Widmer4, Daniela Casoni5, Lukas Andereggen1,2, Javier Fandino1,2, Serge Marbacher1,2 1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Department for Biomedical Research, Faculty of Medicine, University of Bern Developing and testing endovascular devices for intracranial aneurysm treatment is still of great importance. Most aneurysm models used today miss either the important characteristics of an arterial degenerated wall or the hemodynamics of a true bifurcation. Therefore, we aimed to design a novel arterial pouch bifurcation model in rabbits. Medicine Measurements of Motor Function and Other Clinical Outcome Parameters in Ambulant Children with Duchenne Muscular Dystrophy Sara Nagy1,2, Simone Schmidt1,2, Patricia Hafner1,3, Andrea Klein1,4,5, Daniela Rubino-Nacht1, Vanya Gocheva1, Oliver Bieri6, Carole Vuillerot7, Ulrike Bonati1,2, Dirk Fischer1,2,3 1Division of Pediatric Neurology, University Children's Hospital Basel (UKBB), University of Basel, 2Department of Neurology, University Hospital Basel, University of Basel, 3Division of Neurology, University Clinic of Medicine, Cantonal Hospital Baselland, Bruderholz, 4Division of Pediatric Neurology, CHUV, 5Division of Neuropaediatrics, Inselspital, University Children's Hospital Bern, 6Department of Radiology, Division of Radiological Physics, University Hospital Basel, University of Basel, 7Hospices Civils de Lyon, Hôpital Femme-Mère-Enfant, L'Escale, Service de Médecine Physique et de Réadaptation Pédiatrique The aim of this study is to present the most reliable clinical outcome measures and their correlations with quantitative muscle MRI in ambulant patients with Duchenne muscular dystrophy. Biology Cystometric and External Urethral Sphincter Measurements in Awake Rats with Implanted Catheter and Electrodes Allowing for Repeated Measurements Elena E. Foditsch1,2, Karin Roider1,2, Andrea M. Sartori3,4,5, Thomas M. Kessler5, Sabik Raj Kayastha6, Ludwig Aigner2, Marc P. Schneider7 1Department of Urology, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, 2Institute of Molecular Regenerative Medicine, Spinal Cord Injury and Tissue Regeneration Center Salzburg, Paracelsus Medical University, 3Brain Research Institute, University of Zürich, 4Department of Health Sciences and Technology, Swiss Federal Institute of Technology Zürich, 5Neuro-Urology, Spinal Cord Injury Center & Research, University of Zürich, Balgrist University Hospital, 6Department of Orthopaedics and Traumatology, Dhulikhel Hospital, Kathmandu University Hospital, 7Department of Urology, Inselspital, Bern University Hospital This protocol first describes the surgical procedure of the permanent implantation of a urinary bladder catheter combined with external urethral sphincter electrodes, and second, the measurement of the function of the urinary bladder and external urethral sphincter in implanted awake animals. Medicine Discovering Middle Ear Anatomy by Transcanal Endoscopic Ear Surgery: A Dissection Manual Lukas Anschuetz1,2, Livio Presutti2, Daniele Marchioni3, Marco Bonali2, Wilhelm Wimmer1,4, Domenico Villari2, Marco Caversaccio1,4 1Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, 2Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital of Modena, 3Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital of Verona, 4Artificial Hearing Research, Artorg Center for Biomedical Engineering, University of Bern The aim of this article is to describe the methodology of exclusively endoscopic cadaveric middle ear dissection. Moreover, we aim to provide a comprehensive guide to endoscopic middle ear anatomy. Medicine In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy Despina Kokona1, Joël Jovanovic1, Andreas Ebneter1, Martin S. Zinkernagel1 1Department of Ophthalmology and Department of Clinical Research, Bern University Hospital and University of Bern This protocol describes how high-resolution imaging techniques such as spectral domain optical coherence tomography and scanning laser ophthalmoscopy can be utilized in small rodents, using an ophthalmic imaging platform system, to obtain information on retinal thickness and microglial cell distribution, respectively. Neuroscience Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique Edin Nevzati1,2, Jeannine Rey2, Daniel Coluccia1,2, Donato D'Alonzo1,2, Basil Grüter1,2, Luca Remonda3, Javier Fandino1,2, Serge Marbacher1,2 1Department of Neurosurgery, Kantonsspital Aarau, 2Neuro Lab, Research Group for Experimental Neurosurgery and Neurocritical Care, Department of Intensive Care Medicine, University Hospital and University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau Reproducable experimental animal models are needed for the testing of novel embolization materials, which have been designed to treat endovascular occlusion of intracranial aneurysms (IA). The present study aims to develop a safe and standardized surgical technique for stent assisted embolization of saccular aneurysms in a rat animal model. Medicine The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects Lukas Andereggen3,4,5, Volker Neuschmelting1,6, Michael von Gunten7, Hans Rudolf Widmer5, Jukka Takala1, Stephan M. Jakob1, Javier Fandino1,2, Serge Marbacher1,2 1Department of Intensive Care Medicine, University and Bern University Hospital (Inselspital), 2Department of Neurosurgery, Kantonsspital Aarau, 3 The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures — subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.
