Lausanne University Hospital 7 articles published in JoVE Medicine Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy Martino Guiotto*1,4, Silvia Palombella*2, Stefania Brambilla2, Lee Ann Applegate3, Mathis Riehle4, Andrew Hart4, Wassim Raffoul1, Pietro Giovanni di Summa1 1Department of Plastic, Reconstructive and Hand Surgery, Centre Hospitalier Universitaire Vaudois (CHUV, 2 Xenogeneic (chemical or animal-derived) products introduced in the cell therapy preparation/manipulation steps are associated with an increased risk of immune reactivity and pathogenic transmission in host patients. Here, a complete xenogeneic-free method for the isolation and in vitro expansion of human adipose-derived stem cells is described. Genetics Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR Antonio Mancarella1, Francesco A. Procopio1, Tilmann Achsel2, Elisa De Crignis3, Brian T. Foley4, Giampietro Corradin5, Claudia Bagni2, Giuseppe Pantaleo1, Cecilia Graziosi1 1Division of Immunology and Allergy, Lausanne University Hospital, 2Department of Fundamental Neuroscience, University of Lausanne, 3Department of Biochemistry, Erasmus Medical Center, 4Theoretical Biology and Biophysics Group, Los Alamos National Laboratories, 5Department of Biochemistry, University of Lausanne RNA hairpins and loops can function as primers for reverse transcription (RT) in absence of sequence-specific primers, interfering with the study of overlapping antisense transcripts. We have developed a technique able to identify strand-specific RNA, and we have used it to study HIV-1 antisense protein ASP. Behavior Supramaximal Intensity Hypoxic Exercise and Vascular Function Assessment in Mice Jessica Lavier1,2, Manon Beaumann3, Steeve Ménetrey3, Lucia Mazzolai1, Anne-Christine Peyter3, Maxime Pellegrin1, Grégoire P. Millet2 1Division of Angiology, Heart and Vessel Department, Lausanne University Hospital (CHUV), 2Institute of Sport Sciences, Faculty of Biology and Medicine, University of Lausanne, 3Neonatal Research Laboratory, Clinic of Neonatology, Department Woman-Mother-Child, Lausanne University Hospital High-intensity training in hypoxia is a protocol that has been proven to induce vascular adaptations potentially beneficial in some patients and to improve athletes' repeated sprint ability. Here, we test the feasibility of training mice using that protocol and identify those vascular adaptations using ex vivo vascular function assessment. Medicine Measurements of Motor Function and Other Clinical Outcome Parameters in Ambulant Children with Duchenne Muscular Dystrophy Sara Nagy1,2, Simone Schmidt1,2, Patricia Hafner1,3, Andrea Klein1,4,5, Daniela Rubino-Nacht1, Vanya Gocheva1, Oliver Bieri6, Carole Vuillerot7, Ulrike Bonati1,2, Dirk Fischer1,2,3 1Division of Pediatric Neurology, University Children's Hospital Basel (UKBB), University of Basel, 2Department of Neurology, University Hospital Basel, University of Basel, 3Division of Neurology, University Clinic of Medicine, Cantonal Hospital Baselland, Bruderholz, 4Division of Pediatric Neurology, CHUV, 5Division of Neuropaediatrics, Inselspital, University Children's Hospital Bern, 6Department of Radiology, Division of Radiological Physics, University Hospital Basel, University of Basel, 7Hospices Civils de Lyon, Hôpital Femme-Mère-Enfant, L'Escale, Service de Médecine Physique et de Réadaptation Pédiatrique The aim of this study is to present the most reliable clinical outcome measures and their correlations with quantitative muscle MRI in ambulant patients with Duchenne muscular dystrophy. Neuroscience Endoscopic Endonasal Trans-sphenoidal Approach: Minimally Invasive Surgery for Pituitary Adenomas Mahmoud Messerer1, Giulia Cossu1, Mercy George2, Roy Thomas Daniel1 1Department of Neurosurgery, University Hospital of Lausanne, 2Department of Otorhinolaryngology, University Hospital of Lausanne The aim of this paper is to describe the different steps of the endoscopic endonasal approach to the sella turcica. Engineering Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films Ruslan Cusnir1, Philipp Steinmann2, Marcus Christl3, François Bochud1, Pascal Froidevaux1 1Institute of Radiation Physics, Lausanne University Hospital, 2Federal Office of Public Health, Bern, Switzerland, 3Laboratory of Ion Beam Physics, ETH Zurich The technique of diffusive gradients in thin films (DGT) is proposed for speciation studies of plutonium. This protocol describes diffusion experiments probing the behavior of Pu(IV) and Pu(V) in presence of organic matter. DGTs deployed in a karstic spring allow assessment of the bioavailability of Pu. Medicine Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement Alban Longchamp*1, Florent Allagnat*2, Xavier Berard3, Florian Alonso2, Jacques-Antoine Haefliger2, Sébastien Deglise*4, Jean-Marc Corpataux*4 1 The mechanisms leading to the development of intimal hyperplasia (IH) and vein graft failure are still poorly understood. This study describes an ex vivo system to perfuse human veins under controlled flow and pressure. Furthermore the efficiency of external mesh reinforcement to limit the development of IH was evaluated.