University of Zurich & Zurich-Basel Plant Science Center 1 article published in JoVE Biology Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types Ana Marcela Florez Rueda1, Ueli Grossniklaus1, Anja Schmidt1 1University of Zürich & Zürich-Basel Plant Science Center Here we present a protocol for laser-assisted microdissection of specific plant cell types for transcriptional profiling. While the protocol is suitable for different species and cell types, the focus is on highly inaccessible cells of the female germline important for sexual and apomictic reproduction in the crucifer genus Boechera.