Philip Morris Products S.A. 3 articles published in JoVE Biochemistry Shotgun Lipidomics of Rodent Tissues Oksana Lavrynenko1, Sophie Dijon1, Björn Titz1, Nikolai V. Ivanov1 1PMI R&D, Philip Morris Products SA Shotgun mass spectrometry-based lipidomics delivers a sensitive quantitative snapshot of a broad spectrum of lipid classes simultaneously in a single measurement from various rodent tissues. Engineering Use of Capillary Aerosol Generator in Continuous Production of Controlled Aerosol for Non-Clinical Studies Didier Goedertier1, Sandra Schorderet Weber1, Francesco Lucci1, Tom Lee2, Wei Teck Tan2, Falk Radtke1, Subash Krishnan2, Patrick Vanscheeuwijck1, Arkadiusz K. Kuczaj1,3, Julia Hoeng1 1PMI R&D, Philip Morris Products S.A., 2PMI R&D, Philip Morris Research Laboratories Pte. Ltd., 3Applied Mathematics, University of Twente The protocol describes the settings and use of a capillary aerosol generator for continuous production of controlled aerosol from a multispecies liquid solution, suitable for steady large-volume aerosol delivery (e.g., in vivo inhalation studies). Bioengineering Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke Diana Kuehn1, Shoaib Majeed1, Emmanuel Guedj1, Remi Dulize1, Karine Baumer1, Anita Iskandar1, Stephanie Boue1, Florian Martin1, Radina Kostadinova1, Carole Mathis1, Nikolai V. Ivanov1, Stefan Frentzel1, Julia Hoeng1, Manuel C. Peitsch1 1Biological Systems Research, Philip Morris International R&D, Philip Morris Products S.A. The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.
