University Hospital Bonn 9 articles published in JoVE Neuroscience Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration Jeffrey D. Messinger1, Max Brinkmann2, James A. Kimble1, Andreas Berlin1, K. Bailey Freund3,4, Gregory H. Grossman5, Thomas Ach*6, Christine A. Curcio*1 1Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham Heersink School of Medicine, 2Department of Ophthalmology, University Hospital of Zurich, 3Vitreous Retina Macula Consultants of New York, 4Department of Ophthalmology, New York University Grossman School of Medicine, 5Advancing Sight Network, 6Department of Ophthalmology, University Hospital Bonn Laboratory assays can leverage prognostic value from the longitudinal optical coherence tomography (OCT)-based multimodal imaging of age-related macular degeneration (AMD). Human donor eyes with and without AMD are imaged using OCT, color, near-infrared reflectance scanning laser ophthalmoscopy, and autofluorescence at two excitation wavelengths prior to tissue sectioning. Medicine Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation Heidi Ehrentraut*1, Christina K. Weisheit*1, Stilla Frede1, Tobias Hilbert1 1Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn Presented here is a step-by-step procedure to induce acute lung injury in mice by direct intratracheal lipopolysaccharide instillation and to perform FACS analysis of blood samples, bronchoalveolar lavage fluid, and lung tissue. Minimal invasiveness, simple handling, good reproducibility, and titration of disease severity are advantages of this approach. Medicine Evaluation of Patients' Posture and Gait Profile After Lumbar Fusion Surgery by Video Rasterstereography and Treadmill Gait Analysis Sebastian Scheidt1,2, Ulf Krister Hofmann2, Falk Mittag2 1Department of Orthopedics and Trauma Surgery, University Hospital Bonn, 2Department of Orthopedic Surgery, University Hospital Tuebingen Here, we present a protocol to analyze the posture and the gait of patients after lumbar fusion surgery by means of high-resolution video rasterstereography and a treadmill equipped with an integrated sensor mat. Allowing critical functional postoperative evaluation on a less subjective level may enhance accuracy and reliability of indication for surgery. Medicine Vagus Nerve Stimulation As an Adjunctive Neurostimulation Tool in Treatment-resistant Depression Sebastian Moeller1,2, Caroline Lücke2, Christian Heinen3, Bettina H. Bewernick2,4, Merve Aydin2, Alexandra P. Lam2, Teja W. Grömer5, Alexandra Philipsen2, Helge H.O. Müller2 1Universitätsklinik für Psychiatrie und Psychotherapie, Carl von Ossietzky Universität Oldenburg, 2Universitätsklinikum Bonn AöR, Klinik und Poliklinik für Psychiatrie, 3Universitätsklinik für Neurochirurgie am Evangelischen Krankenhaus Oldenburg, Carl von Ossietzky Universität Oldenburg, 4Klinik für Neurodegenerative Erkrankungen und Gerontopsychiatrie, Abteilung für medizinische Psychologie, Klinik für Psychiatrie und Psychotherapie, Universitätsklinikum Bonn, 5Psychiatrische und Psychotherapeutische Klinik, Universitätsklinikum Erlangen Vagus nerve stimulation (VNS) has been shown to be effective as an adjunct treatment for treatment-resistant depression (TRD). VNS leads to antidepressive and antisuicidal effects and quality of life improvements. This protocol offers a step-by-step guide to managing and adjusting a vagus nerve stimulator for efficient treatment of TRD. Medicine A Closed-chest Model to Induce Transverse Aortic Constriction in Mice Lars Eichhorn*1, Christina Katharina Weisheit*1, Christopher Gestrich2, Konrad Peukert1, Georg Daniel Duerr2, Muhammad Ajmal Ayub3, Felix Erdfelder1, Florian Stöckigt4 1Department of Anaesthesiology, University Hospital Bonn, 2Department of Cardiac Surgery, University Hospital Bonn, 3Institute of Physiology 2, University of Bonn, 4Department of Medicine - Cardiology, University Hospital Bonn Here, we present a protocol of Transverse Aortic constriction (TAC) via a lateral thoracotomy. This technique is a minimally invasive, closed chest surgical procedure aiming to simulate pressure overload and heart failure in mice utilizing standard TAC laboratory settings. Medicine Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction Gunnar T.R. Hischebeth1, Sascha Gravius2, Johanna K. Buhr2, Ernst Molitor1, Matthias D. Wimmer2, Achim Hoerauf1, Isabelle Bekeredjian-Ding3, Thomas M. Randau2 1Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, 2Department of Orthopaedics and Trauma Surgery, University Hospital Bonn, 3Division of EU Cooperation/Microbiology, Paul-Ehrlich-Institute Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described. Cancer Research Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples Maximilian Boesch*1,2,3, Elisabeth Hoflehner2,3, Dominik Wolf2,3,4, Guenther Gastl2, Sieghart Sopper*2,3 1Institute of Immunobiology, Kantonsspital St. Gallen, 2Internal Medicine V, Medical University of Innsbruck, 3Tyrolean Cancer Research Institute (TKFI), 4Medical Clinic III, Oncology, Hematology, Immunoncology and Rheumatology, University Clinic Bonn (UKB) Methods allowing the characterization and isolation of stem cell populations from biological samples are critical for the advance of stem cell-targeted treatments in cancer and beyond. Here, we provide a detailed protocol for cancer stem cell isolation using the dye-triggered side population phenotype. Medicine A Model to Simulate Clinically Relevant Hypoxia in Humans Lars Eichhorn1, Florian Kessler1, Volker Böhnert2, Felix Erdfelder1, Anja Reckendorf3, Rainer Meyer4, Richard K. Ellerkmann1 1Department of Anaesthesiology and Intensive Care Medicine, University Hospital of Bonn, 2Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn, 3Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, 4Institute of Physiology 2, University of Bonn Hypoxia simulation in humans has usually been performed by inhaling hypoxic gas mixtures. For this study, apneic divers were used to simulate dynamic hypoxia in humans. Additionally, physiological changes in desaturation and re-saturation kinetics were evaluated with non-invasive tools such as Near-Infrared-Spectroscopy (NIRS) and peripheral oxygenation saturation (SpO2). Medicine The Supraclavicular Fossa Ultrasound View for Central Venous Catheter Placement and Catheter Change Over Guidewire Se-Chan Kim1, Christian Klebach1, Ingo Heinze1, Andreas Hoeft1, Georg Baumgarten1, Stefan Weber1 1Department of Anesthesiology and Intensive Care Medicine, University Hospital Bonn The overall goal is to enable clinicians to insert a central venous catheter under real-time ultrasound guidance via the right supraclavicular fossa view of the lower part of the superior vena cava. This view is useful for different catheter insertion sites, thrombosis detection before insertion and ultrasound guided correction of misplaced catheters.