Leibniz Institute for Neurobiology 4 articles published in JoVE Medicine Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease Martin Wagner1, Claudia Baer1, Werner Zuschratter2, Monika Riek-Burchardt3, Christian Deffge1, Soenke Weinert1, Jerry C Lee4, Ruediger C Braun-Dullaeus1, Joerg Herold1 1Department of Cardiology and Angiology, University of Magdeburg, 2Leibniz Institute for Neurobiology, 3Institute of Molecular and Clinical Immunology, University of Magdeburg, 4 Monocytes are important mediators of arteriogenesis in the context of peripheral arterial disease. Using a basement membrane-like matrix and intravital microscopy, this protocol investigates monocyte homing and tumor-related angiogenesis after monocyte injection in the femoral artery ligation murine model. Neuroscience High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning Angela Kolodziej1, Karl-Heinz Smalla1, Sandra Richter2, Alexander Engler1, Rainer Pielot1,3, Daniela C. Dieterich3, Wolfgang Tischmeyer1, Michael Naumann2, Thilo Kähne2 1Leibniz Institute for Neurobiology (LIN), 2Institute of Experimental Internal Medicine, Medical School, Otto von Guericke University Magdeburg, 3Institute of Pharmacology and Toxicology, Medical School, Otto von Guericke University The identification of molecules and pathways controlling synaptic plasticity and memory is still a major challenge in neuroscience. Here, a workflow is described addressing the relative quantification of synaptic proteins supposedly involved in the molecular reorganization of synapses during learning and memory consolidation in an auditory learning paradigm. Behavior Combined Shuttle-Box Training with Electrophysiological Cortex Recording and Stimulation as a Tool to Study Perception and Learning Max F.K. Happel1,2, Matthias Deliano*1, Frank W. Ohl*1,2,3 1Leibniz Institute for Neurobiology, Magdeburg, Germany, 2Otto-von-Guericke University, Magdeburg, Germany, 3Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany Shuttle-box avoidance learning is well-established in behavioral neuroscience. This protocol describes how shuttle-box learning in rodents can be combined with site-specific electrical intracortical microstimulation (ICMS) and simultaneous chronical in vivo recordings as a tool to study multiple aspects of learning and perception. Neuroscience Isolation of CA1 Nuclear Enriched Fractions from Hippocampal Slices to Study Activity-dependent Nuclear Import of Synapto-nuclear Messenger Proteins Pingan Yuanxiang1, Sujoy Bera1, Anna Karpova1, Michael R. Kreutz1, Marina Mikhaylova1,2 1RG Neuroplasticity, Leibniz Institute for Neurobiology, 2Department of Cell Biology, Utrecht University We provide a detailed protocol for induction of long-term potentiation in the CA1 region of the hippocampus and the subsequent isolation of nuclear enriched fractions from the tetanized area of the slice. This approach can be used to determine activity dependent nuclear protein import in cellular models of learning and memory.
