Klinikum der Universitat Munchen 1 article published in JoVE Medicine Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery Christoph Emontzpohl1,2, David Simons3, Sandra Kraemer4, Andreas Goetzenich4, Gernot Marx1, Jürgen Bernhagen5,6, Christian Stoppe1 1Department of Intensive Care Medicine, University Hospital Aachen, 2Institute of Biochemistry and Molecular Biology, University Hospital Aachen, 3Department of Radiology, German Cancer Research Center, 4Department of Thoracic and Cardiovascular Surgery, University Hospital Aachen, 5Department of Vascular Biology, Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, 6Deutsches Zentrum für Herz-/Kreislaufkrankheiten (DZHK), Munich Heart Alliance Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.
