Humboldt University of Berlin 7 articles published in JoVE Medicine Magnetic Resonance Imaging of Multiple Sclerosis at 7.0 Tesla Sonia Waiczies*1, Antje Els*1, Joseph Kuchling*2,3,4, Karin Markenroth Bloch5, Anna Pankowska6,7, Helmar Waiczies8, Carl Herrmann1, Claudia Chien2,3, Carsten Finke4,9, Friedemann Paul2,3,4, Thoralf Niendorf1,2,8 1Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 2Experimental and Clinical Research Center, Max Delbrueck Center for Molecular Medicine and Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3NeuroCure Clinical Research Center, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 4Department of Neurology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 5The Swedish National 7T Facility, Lund University Bioimaging Center, Lund University, 6Department of Radiography, Medical University of Lublin, 7ECOTECH-COMPLEX, Maria Curie-Skłodowska University, 8MRI.TOOLS GmbH, 9Berlin School of Mind and Brain, Humboldt-Universität zu Berlin Here, we present a protocol to acquire magnetic resonance (MR) images of multiple sclerosis (MS) patient brains at 7.0 Tesla. The protocol includes preparation of the setup including the radio-frequency coils, standardized interview procedures with MS patients, subject positioning in the MR scanner and MR data acquisition. Biology Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish Savvas J. Constantinou1, Linh Nguyen2, Frank Kirschbaum2, Vielka L. Salazar3, Jason R. Gallant1 1Department of Integrative Biology, Michigan State University, 2Faculty of Life Sciences, Unit of Biology and Ecology of Fishes, Humboldt University, 3Department of Biology, Cape Breton University Here, a protocol is presented to produce and rear CRISPR/Cas9 genome knockout electric fish. Outlined in detail are the required molecular biology, breeding, and husbandry requirements for both a gymnotiform and a mormyrid, and injection techniques to produce Cas9-induced indel F0 larvae. Behavior Eye Tracking During Visually Situated Language Comprehension: Flexibility and Limitations in Uncovering Visual Context Effects Camilo Rodriguez Ronderos1, Katja Münster1, Ernesto Guerra2, Helene Kreysa3, Alba Rodríguez1,4, Julia Kröger4, Thomas Kluth4, Michele Burigo4, Dato Abashidze5, Eva Nunnemann4, Pia Knoeferle1,6,7 1Institute of German Studies and Linguistics, Humboldt-Universität zu Berlin, 2Center for Advanced Research in Education – CIAE, Universidad de Chile, 3Institute of Psychology, Friedrich Schiller University Jena, 4Cognitive Interaction Technology Excellence Cluster (CITEC), Faculty of Linguistics and Literature, Bielefeld University, 5Department of Education, Concordia University, 6Berlin School of Mind and Brain, 7Einstein Center for Neurosciences Berlin The present article reviews an eye-tracking methodology for studies on language comprehension. To obtain reliable data, key steps of the protocol must be followed. Among these are the correct set-up of the eye tracker (e.g., ensuring good quality of the eye and head images) and accurate calibration. Neuroscience Noninvasive EEG Recordings from Freely Moving Piglets Nora V. de Camp1,2, Silke Dietze1, Markus Klaßen3, Jürgen Bergeler1 1Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Veterinary Medicine, Free University Berlin, 2Department of Biology, Behavioral Physiology, Humboldt University Berlin, 3Lehr- und Versuchsanstalt für Viehhaltung Hofgut Neumühle Here, we present a protocol to record telemetric electroencephalograms (EEGs) from freely moving piglets directly in the pigpen without the use of a sedative, making it possible to record typical EEG patterns during non-REM sleep, like spindle bursts. Biochemistry Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers Ralph Menzel1, Rainer Nehring1, Dilara Simsek1, Liliane Ruess1 1Ecology, Institute of Biology, Humboldt-Universität zu Berlin The fatty acid trophic marker approach, i.e., the assimilation of fatty acids as entire molecule and transfer into consumer tissue with no or minor modification, is hampered by knowledge gaps in fatty acid metabolism of small soil invertebrates. Isotopologue profiling is provided as a valuable tool to disentangle trophic interactions. Neuroscience Visualization of Cortical Modules in Flattened Mammalian Cortices Simon M. Lauer*1, Undine Schneeweiß1, Michael Brecht1,2,3, Saikat Ray*1 1Bernstein Center for Computational Neuroscience, Humboldt University of Berlin, 2NeuroCure Cluster of Excellence, 3German Center for Neurodegenerative Diseases This article describes a detailed methodology to obtain flattened tangential sections from mammalian cortices and visualize cortical modules using histochemical and immunohistochemical methods. Chemistry Retropinacol/Cross-pinacol Coupling Reactions - A Catalytic Access to 1,2-Unsymmetrical Diols Ulf Scheffler1, Rainer Mahrwald1 1Department of Chemistry, Humboldt University of Berlin A novel account for the synthesis of unsymmetrical 1,2-diols based on a retropinacol/cross-pinacol coupling mechanism is described. Due to the catalytic execution of this reaction a considerable improvement compared to conventional cross-pinacol couplings is achieved.
