Xtal Concepts GmbH 1 article published in JoVE Biochemistry Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering Daniela Baitan1,2, Robin Schubert2,3, Arne Meyer1, Karsten Dierks1, Markus Perbandt2,3, Christian Betzel2,3 1Xtal Concepts GmbH, 2Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation c/o DESY, University of Hamburg, 3The Hamburg Center for Ultrafast Imaging, University of Hamburg Here we present a protocol for controlled production of protein microcrystals. The process uses an automated device allowing controlled manipulation of several crystallization parameters. The protein crystallization is carried-out by controlled and automated addition of crystallization solutions while monitoring and investigating the radius distribution of particles in the crystallization droplet.
