Max Planck Institute for Biology of Ageing 2 articles published in JoVE Developmental Biology A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga Chlamydomonas reinhardtii Umarah Mubeen1, Lais Albuquerque Giraldi1, Jessica Jüppner1, Patrick Giavalisco1,2 1Max Planck Institute of Molecular Plant Physiology, 2Max Planck Institute of Biology of Ageing System-wide analysis of multiple biomolecules is crucial to gain functional and mechanistic insights into biological processes. Hereby, an extensive protocol is described for high throughput extraction of lipids, metabolites, proteins and starch from a single sample harvested from synchronized Chlamydomonas culture. Biology A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri) Joanna Dodzian1,3, Sam Kean1, Jens Seidel1, Dario Riccardo Valenzano1,2 1Max Planck Institute for the Biology of Ageing, 2CECAD, University of Cologne, 3International Institute of Molecular and Cell Biology in Warsaw Laboratory housing of turquoise killifish can be scaled up to house and efficiently raise thousands of individual fish in a centralized water filtration system, employing the same infrastructure used for standard zebrafish facilities. Here we detail a list of standardized procedures that allow efficient killifish maintenance.
