Berlin Institute of Medical Systems Biology of the Max Delbruck Center 1 article published in JoVE Developmental Biology Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development Jaroslav Icha*1, Christopher Schmied*1, Jaydeep Sidhaye1, Pavel Tomancak1, Stephan Preibisch1,2,3, Caren Norden1 1Max Planck Institute of Molecular Cell Biology and Genetics, 2HHMI Janelia Research Campus, 3Berlin Institute of Medical Systems Biology of the Max Delbrück Center Light sheet fluorescence microscopy is an excellent tool for imaging embryonic development. It allows recording of long time-lapse movies of live embryos in near physiological conditions. We demonstrate its application for imaging zebrafish eye development across wide spatio-temporal scales and present a pipeline for fusion and deconvolution of multiview datasets.
