Deutsches Rheuma-Forschungszentrum (DRFZ) 1 article published in JoVE Cancer Research Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps Fabian L. Kriegel1,2, Ralf Köhler2, Jannike Bayat-Sarmadi2, Simon Bayerl3, Anja E. Hauser2,3, Raluca Niesner2, Andreas Luch*1, Zoltan Cseresnyes*4 1Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), 2Deutsches Rheuma-Forschungszentrum (DRFZ) Berlin, a Leibniz Institute, 3Charité Universitätsmedizin Berlin, 4Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute Here, we provide a workflow that allows the identification of healthy and pathological cells based on their 3-dimensional shape. We describe the process of using 2D projection outlines based on the 3D surfaces to train a Self-Organizing Map that will provide objective clustering of the investigated cell populations.
