Christian-Albrechts-University Kiel 4 articles published in JoVE Neuroscience Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice Gilda Baccini1, Sören Brandt1, Peer Wulff1 1Institute of Physiology, Christian-Albrechts-University Kiel We present a protocol to prepare acute-slices from the dorsal-intermediate hippocampus of mice. We compare this transversal preparation with coronal slicing in terms of quality of recordings and preservation of morphological features of recorded neurons. Engineering Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet Judith Golda1, Kerstin Sgonina1, Julian Held2, Jan Benedikt1, Volker Schulz-von der Gathen2 1Experimental Plasma Physics, Kiel University, 2Experimental Physics II, Ruhr-University Bochum This protocol is presented to characterize the setup, handling, and application of the COST-Jet for the treatment of diverse surfaces such as solids and liquids. Genetics Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach Tanita Wein*1, Fenna T. Stücker*1, Nils F. Hülter1, Tal Dagan1 1Institute of Microbiology, Kiel University Our experimental approach provides a strategy to follow plasmid abundance and antibiotic resistance over time in bacterial populations. Environment Calibrated Passive Sampling - Multi-plot Field Measurements of NH3 Emissions with a Combination of Dynamic Tube Method and Passive Samplers Andreas Pacholski1,2 1Institute of Ecology, Assoc. Institute of Environmental and Sustainable Chemistry, Leuphana University, 2Institute of Crop Science and Plant Breeding, Department of Agronomy and Crop Science, Christian-Albrechts-University Kiel Ammonia emissions are a major threat to the environment by eutrophication, soil acidification and fine particle formation and stem mainly from agricultural sources. This method allows ammonia loss measurements in replicated field trials enabling statistical analysis of emissions and of relationships between crop development and emissions.
