University Hospital Tuebingen 4 articles published in JoVE Medicine Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow Antonia Link1, Giorgio Cattaneo2,3, Eduard Brynda4, Tomas Riedel4, Johanka Kucerova4, Christian Schlensak1, Hans Peter Wendel1, Stefanie Krajewski1, Tatjana Michel1 1Department of Thoracic, Cardiac and Vascular Surgery, University Hospital Tuebingen, 2Acandis GmbH, 3Institute of Biomedical Engineering, University of Stuttgart, 4Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic This protocol describes a comprehensive hemocompatibility evaluation of blood-contacting devices using laser-cut neurovascular implants. A flow loop model with fresh, heparinized human blood is applied to mimic blood flow. After perfusion, various hematologic markers are analyzed and compared to the values gained directly after blood collection for hemocompatibility evaluation of the tested devices. Medicine Evaluation of Patients' Posture and Gait Profile After Lumbar Fusion Surgery by Video Rasterstereography and Treadmill Gait Analysis Sebastian Scheidt1,2, Ulf Krister Hofmann2, Falk Mittag2 1Department of Orthopedics and Trauma Surgery, University Hospital Bonn, 2Department of Orthopedic Surgery, University Hospital Tuebingen Here, we present a protocol to analyze the posture and the gait of patients after lumbar fusion surgery by means of high-resolution video rasterstereography and a treadmill equipped with an integrated sensor mat. Allowing critical functional postoperative evaluation on a less subjective level may enhance accuracy and reliability of indication for surgery. Biology In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells Meltem Avci-Adali1, Andreas Behring1, Heidrun Steinle1, Timea Keller1, Stefanie Krajeweski1, Christian Schlensak1, Hans P. Wendel1 1Department of Thoracic, Cardiac, and Vascular Surgery, University Hospital Tuebingen In this video article, we describe the in vitro synthesis of modified mRNA for induction of protein expression in cells. Immunology and Infection Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus Christina Stoeckle1,2, Ioanna A. Rota1, Eva Tolosa3, Christoph Haller4, Arthur Melms5, Eleni Adamopoulou1 1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
