Julius-Maximilians University Wurzburg 1 article published in JoVE Biochemistry Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation Lukas Pekarek1, Stefan Buck1, Neva Caliskan1,2 1Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research), 2Medical Faculty, Julius-Maximilians University Würzburg This protocol presents a complete experimental workflow for studying RNA-protein interactions using optical tweezers. Several possible experimental setups are outlined including the combination of optical tweezers with confocal microscopy.
