German Research Center for Environmental Health 1 article published in JoVE Neuroscience Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes Bob A. Hersbach1,2,3, Tatiana Simon2, Giacomo Masserdotti1,2 1Institute of Stem Cell Research, Helmholtz Zentrum München, German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich, Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter, Ludwig-Maximilians University Here we describe a detailed protocol to generate highly enriched cultures of astrocytes derived from different regions of the central nervous system of postnatal mice and their direct conversion into functional neurons by the forced expression of transcription factors.
