Sachsisches Krankenhaus Rodewisch 1 article published in JoVE Immunology and Infection Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test Julia Häring*1, Tanja Michel*1, Matthias Becker1, Daniel Junker1, Tatia Tchitchagua2, Olaf Leschnik2, Berit Lange3,4, Stefanie Castell3,4, Gérard Krause3,4, Monika Strengert3, Alex Dulovic1, Nicole Schneiderhan-Marra1 1NMI Natural and Medical Sciences Institute at the University of Tübingen, 2Department of Neurology, Sächsisches Krankenhaus Rodewisch, 3Department of Epidemiology, Helmholtz Centre for Infection Research, 4German Centre for Infection Research (DZIF) A three-channel dual-reporter fluorescence flow analysis system was used to develop a bead-based multiplex immunoassay that simultaneously evaluates serum samples for IgG and IgM elicited against multiple antigens of different Borrelia species that cause Lyme borreliosis in Europe and North America.
