Heinrich Heine University Duesseldorf 1 article published in JoVE Neuroscience Simultaneous Isolation of Principal Central Nervous System-Resident Cell Types from Adult Autoimmune Encephalomyelitis Mice Christina B. Schroeter*1, Antonia Henes*1, Anna Vogelsang1, Alexander M. Herrmann1, Stefanie Lichtenberg1, Derya Cengiz1, Vera Dobelmann1, Niklas Huntemann1, Christopher Nelke1, Susann Eichler2, Philipp Albrecht1, Sven G. Meuth*1, Tobias Ruck1 1Department of Neurology, Medical Faculty, Heinrich Heine University Duesseldorf, 2Department of Neurology, Institute of Translational Neurology, University Hospital Muenster To date, protocols for the simultaneous isolation of all principal central nervous system-resident cell types from the same mouse are an unmet demand. The protocol shows a procedure applicable in naïve and experimental autoimmune encephalomyelitis mice to investigate complex cellular networks during neuroinflammation and simultaneously reduce the required mice numbers.
