CIBER de Enfermedades Cardiovasculares 2 articles published in JoVE Developmental Biology En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos Tamara Gonzalez-Costa1,2, Jose Luis de la Pompa1,2, Joaquim Grego-Bessa1,2 1Intercellular Signalling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Ciber de Enfermedades Cardiovasculares Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development. Immunology and Infection An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells Raquel Toribio-Fernandez1, Virginia Zorita1, Beatriz Herrero-Fernandez2, Jose M. Gonzalez-Granado1,2,3 1LamImSys Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 2LamImSys Lab, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 3CIBER de Enfermedades Cardiovasculares Here, we present a protocol for the in vivo determination of naïve CD4 T cell (T cell) activation, proliferation, and Th1 differentiation induced by GM-CSF bone marrow (BM)-derived dendritic cells (DCs). In addition, this protocol describes BM and T-cell isolation, DC generation, and DC and T-cell adoptive transfer.
