Centro Nacional de Investigaciones Cardiovasculares (CNIC) 2 articles published in JoVE Developmental Biology En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos Tamara Gonzalez-Costa1,2, Jose Luis de la Pompa1,2, Joaquim Grego-Bessa1,2 1Intercellular Signalling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Ciber de Enfermedades Cardiovasculares Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development. Developmental Biology Live Imaging of Early Cardiac Progenitors in the Mouse Embryo Miquel Sendra1, Jorge Mañes1, Jorge N. Domínguez2,3, Miguel Torres1 1Cardiovascular Regeneration Program, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Jaén, 3Fundación Medina, Granada We present a detailed protocol for mouse embryo culture and imaging that enables 3D + time imaging of cardiac progenitor cells. This video-toolkit addresses the key skills required for successful live imaging otherwise hard to acquire from text-only publications.
