Universite de Strasbourg - CNRS 4 articles published in JoVE Biochemistry MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria Noémie Mercier1, Karine Prévost2, Eric Massé2, Pascale Romby1, Isabelle Caldelari1, David Lalaouna1 1Université de Strasbourg, CNRS, 2Department of Biochemistry, Université de Sherbrooke MAPS technology has been developed to scrutinize the targetome of a specific regulatory RNA in vivo. The sRNA of interest is tagged with a MS2 aptamer enabling the co-purification of its RNA partners and their identification by RNA sequencing. This modified protocol is particularly suited for Gram-positive bacteria. Developmental Biology Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo Renee Wei-Yan Chow*1,2,3,4, Paola Lamperti*1,2,3,4, Emily Steed1,2,3,4, Francesco Boselli1,2,3,4, Julien Vermot1,2,3,4 1Institut de Génétique et de Biologie Moléculaire et Cellulaire, 2UMR7104, Centre National de la Recherche Scientifique, 3U964, Institut National de la Santé et de la Recherche Médicale, 4Université de Strasbourg This protocol describes a method for the photoconversion of Kaede fluorescent protein in endocardial cells of the living zebrafish embryo that enables the tracking of endocardial cells during atrioventricular canal and atrioventricular heart valve development. Medicine Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy Reza Tavakoli1, Simona Nemska1,2, Peiman Jamshidi3, Max Gassmann1, Nelly Frossard2 1Institute of Veterinary Physiology and Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, 2Laboratoire d'Innovation Thérapeutique, UMR7200, Université de Strasbourg - CNRS, 3Department of Cardiology, Canton Hospital Lucerne The aim of this protocol is to describe step-by-step the technique of minimally invasive transverse aortic constriction (TAC) in mice. By elimination of intubation and ventilation which are mandatory for the commonly used standard procedure, minimally invasive TAC simplifies the operative procedure and reduces the strain put on the animal. Bioengineering Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening Viktoria Wollrab1,2, David Caballero1,2, Raghavan Thiagarajan1,2, Daniel Riveline1,2 1Laboratory of Cell Physics, Institut de Science et d'Ingénierie Supramoléculaires (ISIS), CNRS and Université de Strasbourg, 2Development and Stem Cells Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS and Université de Strasbourg We report a device and a new method to study cells and embryos. Single cells are precisely ordered in microcavity arrays. Their 3D confinement is a step towards 3D environments encountered in physiological conditions and allows organelle orientation. By controlling cell shape, this setup minimizes variability reported in standard assays.