Assistance-Publique-Hospitaux-de-Marseille 2 articles published in JoVE Biology Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation Dehia Sahmi-Bounsiar1, Paulo Victor de Miranda Boratto2, Graziele Pereira Oliveira2, Jacques Yaacoub Bou Khalil1, Bernard La Scola1,2, Julien Andreani2 1Institut Hospitalo-Universitaire (IHU) - Méditerranée Infection, 2Microbes, Evolution, Phylogeny and Infection (MEΦI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198, Assistance Publique - Hôpitaux de Marseille (AP-HM) Here, we describe a single cell micro-aspiration method for the separation of infected amoebae. In order to separate viral subpopulations in Vermamoeba vermiformis infected by Faustoviruses and unknown giant viruses, we developed the protocol detailed below and demonstrated its ability to separate two low-abundance novel giant viruses. Bioengineering An Efficient and Reproducible Protocol for Distraction Osteogenesis in a Rat Model Leading to a Functional Regenerated Femur Martine Pithioux*1,2, Flavy Roseren*1,2, Christian Jalain4, Franck launay1,3, Philippe Charpiot5, Patrick chabrand1,2, Sandrine Roffino1, Edouard Lamy1,6 1CNRS, ISM, Inst Movement Sci, Aix Marseille Univ, 2Sainte-Marguerite Hospital, Institute for Locomotion, Department of Orthopaedics and Traumatology, APHM, 3Sainte-Marguerite Hospital, Institute for Locomotion, Department of Peadiatric Orthopaedics, APHM, 4Ecole centrale de Marseille, 5Faculté de Pharmacie, Laboratoire de Biochimie, 6Service Central de la Qualité et de l'Information Pharmaceutiques, APHM This study describes a reproducible and detailed protocol using a newly developed external fixator for distraction osteogenesis (DO) in a femoral rat model which permits physiological weight-bearing by the animal after removal of the external fixator.
