TIMC-IMAG 1 article published in JoVE Biology Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture Cedric Allier1, Romaric Vincent1, Fabrice Navarro2, Mathilde Menneteau2, Lamya Ghenim3,4, Xavier Gidrol3, Thomas Bordy1, Lionel Hervé1, Olivier Cioni1, Sabine Bardin5, Michel Bornens5, Yves Usson4,6, Sophie Morales1 1CEA, LETI, DTBS, LISA, Université Grenoble Alpes, 2CEA, LETI, DTBS, LBAM, Université Grenoble Alpes, 3CEA, INSERM, BIG, Université Grenoble Alpes, 4CNRS, FR CNRS 3425, 5CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport, PSL Research University, Institut Curie, 6TIMC-IMAG Lens-free video microscopy enables us to monitor cell cultures directly inside the incubator. Here we describe the full protocol used to acquire and analyze a 2.7 day long acquisition of cultured HeLa cells, leading to a dataset of 2.2 x 106 measurements of individual cell morphology and 10584 cell cycle tracks.
