IPBS, Universite de Toulouse, CNRS, UPS 4 articles published in JoVE Biology Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging Jérôme Gilleron1, Cindy Meziat1, André Sulen2, Stoyan Ivanov3, Jennifer Jager1, David Estève4, Catherine Muller4, Jean-Francois Tanti1, Mireille Cormont1 1Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 3Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging. Biology Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions Anaïs Bouissou*1, Amsha Proag*1, Marion Portes1, Vanessa Soldan2, Stéphanie Balor2, Christophe Thibault3,4, Christophe Vieu3,4, Isabelle Maridonneau-Parini1, Renaud Poincloux1 1Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, 2METi, 3CNRS, LAAS, 4Univ de Toulouse, INSA Here, we detail the experimental techniques used to evaluate the protrusion forces that podosomes apply on a compliant film, from the preparation of the film to the automated analysis of topographical images. Neuroscience Neuropharmacological Manipulation of Restrained and Free-flying Honey Bees, Apis mellifera Eirik Søvik*1,2, Jenny A. Plath*3,4, Jean-Marc Devaud5, Andrew B. Barron3 1Department of Science and Mathematics, Volda University College, 2Department of Biology, Washington University in St. Louis, 3Department of Biological Sciences, Macquarie University, 4Department of Biology, University of Konstanz, 5Research Center on Animal Cognition, CNRS, Universite de Toulouse This manuscript describes several protocols for administering pharmacological agents to honey bees, including simple noninvasive methods for free-flying bees, as well as more invasive variants that allow precise localized treatment of restrained bees. Cancer Research Operating Procedures of the Electrochemotherapy for Treatment of Tumor in Dogs and Cats Natasa Tozon1, Ursa Lampreht Tratar2, Katarina Znidar3, Gregor Sersa2, Justin Teissie4,5, Maja Cemazar2,3 1Clinic for Surgery and Small Animals, Veterinary Faculty, University of Ljubljana, 2Department of Experimental Oncology, Institute of Oncology Ljubljana, 3Faculty of Health Sciences, University of Primorska, 4IPBS (Institut de Pharmacologie et de Biologie Structurale), CNRS, 5IPBS (Institut de Pharmacologie et de Biologie Structurale), Université de Toulouse Electrochemotherapy is the local treatment of solid tumors. The main mechanism is electroporation-mediated permeabilization of the tumor cells' membrane which enables increased internalization of cytostatics like bleomycin and cisplatin. Thus, antitumor effectiveness is potentiated at the site of the application of electric pulses to the tumors.
