INSERM U1209 2 articles published in JoVE Biochemistry Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment Magali Court1,2, Marie Malier1,2, Arnaud Millet1,2,3 1Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, 2Université Grenoble Alpes, 3Pôle Recherche, Centre Hospitalier Universitaire des Alpes We present a protocol to obtain proteomic signatures of human macrophages and apply this to determination of the impact of a low oxygen environment on macrophage polarization. Biology Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads Angélique Millon-Frémillon*1, Julien Aureille*1, Christophe Guilluy1 1Institute for Advanced Biosciences, Centre de recherche UGA - INSERM U1209 - CNRS UMR Cell surface adhesions are central in mechanotransduction, as they transmit mechanical tension and initiate the signaling pathways involved in tissue homeostasis and development. Here, we present a protocol for dissecting the biochemical pathways that are activated in response to tension, using ligand-coated magnetic microbeads and force application to adhesion receptors.
