University of Nimes 2 articles published in JoVE Neuroscience Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats Martin M. Bertrand1,2, Janet R. Keast1 1Department of Anatomy and Neuroscience, University of Melbourne, 2Department of Visceral Surgery, CHU de Nîmes The major pelvic ganglia contain parasympathetic and sympathetic neurons that innervate pelvic organs. Here we describe a dissection method and provide schematics for identification of these ganglia and their associated nerves. These methods can be applied to experimental manipulation of these ganglia in vivo or removal post-mortem for further study. Biology Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope Vasyl Mytskaniuk*1, Fabrice Bardin*2,3, Hassan Boukhaddaoui1,5, Herve Rigneault4, Nicolas Tricaud1 1INSERM U1051, Institut des Neurosciences de Montpellier (INM), Université de Montpellier, 2Université de Nîmes, 3CNRS, IES, UMR 5214, 4Aix-Marseille Université, CNRS, École Centrale Marseille, Institut Fresnel, UMR 7249, 5Montpellier RIO Imaging (MRI) Coherent anti-Stokes Raman scattering (CARS) microscopy based on inherent vibration of molecule bonds permits label-free chemically selective live cell imaging. This work presents the implementation of a complementary microscopy technique on a standard multiphoton laser scanning microscope based on a femtosecond Ti:sapphire laser and an OPO laser.
