L'atelier du Developpeur 1 article published in JoVE Genetics High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology Béatrice Colin1, Nicolas Rocq2, Benoit Deprez1, Cyril Couturier1 1Institut Pasteur de Lille, Institut National de la Santé et de la Recherche Médicale (Inserm) U1177, Drugs and Molecules for Living Systems, Université de Lille, 2 This protocol describes high-throughput plasmid transfection of mammalian cells in a 384-well plate using acoustic droplet ejection technology. The time-consuming, error-prone DNA dispensing and multiplexing, but also the transfection reagent dispensing, are software-driven and performed by a nanodispenser device. The cells are then seeded in these prefilled wells.
