Inserm U1016 1 article published in JoVE Immunology and Infection Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes Hélène Salmon1,2, Ana Rivas-Caicedo1,2, François Asperti-Boursin1,2, Camille Lebugle1,2, Pierre Bourdoncle1,2, Emmanuel Donnadieu1,2 1Institut Cochin, Université Paris Descartes, CNRS (UMR 8104), 2Inserm, U1016, Paris, France This protocol describes a method to image fluorescent T cells introduced into lymph node slices. The technique permits real-time analyses of T cell migration with traditional widefield fluorescence or confocal microscopes.
