Assistance Publique Hopitaux de Paris 2 articles published in JoVE Biology Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine Mairead Kelly1,2, Elise Dreano1,2, Aurelie Hatton1,2, Agathe Lepissier1,2, Anita Golec1,2, Isabelle Sermet-Gaudelus1,2,3, Iwona Pranke1,2,3 1Institut Necker Enfants Malades, 2Université de Paris, 3Centre de Référence Maladies Rares Mucoviscidose et Maladies apparentées, Assistance Publique Hôpitaux de Paris Here we describe the isolation, amplification, and differentiation of primary human nasal epithelial (HNE) cells at the air-liquid interface and a biobanking protocol allowing to successfully freeze and then thaw amplified HNE. The protocol analyzes electrophysiological properties of differentiated HNE cells and CFTR-related chloride secretion correction upon different modulator treatments. Developmental Biology 2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development Lucile Boutaud*1, Marie Michael*1, Céline Banal2, Damelys Calderon1, Sarah Farcy1, Julie Pernelle1, Nicolas Goudin3, Camille Maillard1, Clémantine Dimartino1, Cécile Deleschaux1, Sébastien Dupichaud4, Corinne Lebreton1, Sophie Saunier1, Tania Attié-Bitach1,5, Nadia Bahi-Buisson1,6, Nathalie Lefort2, Sophie Thomas1 1Imagine Institute, INSERM UMR 1163, Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163, Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163, Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology, APHP- Necker Enfants Malades Hospital We present detailed protocols for the generation and characterization of 2D and 3D human induced pluripotent stem cell (hIPSC)-based models of neocortical development as well as complementary methodologies enabling qualitative and quantitative analysis of primary cilium (PC) biogenesis and function.
